preimmune control rabbit serum  (Qijing Trading Co)

Journal: Journal of Bacteriology

Article Title: Identification and Characterization of a New Ferric Enterobactin Receptor, CfrB, in Campylobacter ▿ †

doi: 10.1128/JB.00478-10

Figure Lengend Snippet: Critical role of CfrB in Campylobacter-host interaction. (A) Effect of the CfrB mutation on colonization of chickens by C. jejuni JL11 and C. coli JL170. The experiment included two groups, and chickens in each group (15 birds) were inoculated with a 1:1 mixture of a specific Campylobacter strain and its isogenic cfrB mutant. Fecal samples were collected by cloacal swabs and used for CFU enumeration. Each bar represents the mean log transformed CFU of each strain in each group at the indicated days postinoculation (DPI). The broken line indicates the limit of detection. (B and C) Inactivation of CfrB abolishes C. coli JL170 colonization of chickens. The experiment included three groups of birds. The first two groups of birds were inoculated with JL170 or its isogenic cfrB mutant (JL170 CfrB−) (B), while the third group was infected with a 1:1 mixture of the two strains (C). For each group, six birds were euthanized and the cecal contents were collected at the indicated DPI. Each symbol indicates the log number of CFU/g of cecal contents for a single chicken. The horizontal bars indicate the means of groups at the indicated times. The broken line indicates the limit of detection. (D) Inhibitory effect of CfrB-specific antiserum on FeEnt-mediated growth promotion in Campylobacter. CfrB antiserum, MOMP antiserum, preimmune serum, or PBS was mixed with JL11 and JL170 cells for a modified growth promotion assay as described in Materials and Methods.

Article Snippet: Prior to the assay, the CfrB-specific rabbit antiserum, the preimmune control rabbit serum, and the major outer membrane protein (MOMP) rabbit antiserum specific for Campylobacter (kindly provided by Qijing Zhang, Iowa State University) were heat inactivated at 56°C for 30 min. To evaluate the inhibitory effect of anti-CfrB serum on CfrB function, 1 ml of MH broth grown log-phase culture of each Campylobacter strain was mixed with 1 ml of either CfrB antiserum, preimmune serum, MOMP antiserum, or phosphate-buffered saline (PBS) and then added to 23 ml of MH agar containing 20 μM DFO and poured into petri dishes for solidification.

Techniques: Mutagenesis, Transformation Assay, Infection, Modification

Journal: Endocrinology

Article Title: Expression and microvillar localization of scavenger receptor, class B, type I (a high density lipoprotein receptor) in luteinized and hormone-desensitized rat ovarian models.

doi: 10.1210/endo.139.6.6056

Figure Lengend Snippet: FIG. 6. Ultrastructural localization of SR-BI in the luteinized ovary. The figure is a low magnification slice through portions of two luteal cells and shows the distribu- tion of 10-nm gold particles representing SR-BI labeling. Most of the gold is associ- ated with cell surface microvilli and mi- crovillar channels and can be found scat- tered throughout this compartment (mvc). An occasional gold particle is also seen within the cell cytoplasm, and these, too, may represent specific labeling for SR-BI, as sections incubated with preimmune sera show little of this staining. The antiserum used was against the carboxyl-terminus of SR-BI (1:100).

Article Snippet: Paraffin-prepared sections were blocked with 5% goat serum and 5% nonfat milk (1 h, 37 C), incubated with primary antibody or preimmune serum (1:1000) overnight at 4 C, and labeled with a standard biotinylated horseradish peroxidase procedure (Vector Laboratories, Burlingame, CA).

Techniques: Labeling, Incubation, Staining

Journal: Endocrinology

Article Title: Expression and microvillar localization of scavenger receptor, class B, type I (a high density lipoprotein receptor) in luteinized and hormone-desensitized rat ovarian models.

doi: 10.1210/endo.139.6.6056

Figure Lengend Snippet: FIG. 7. Localization of SR-BI with mi- crovilli and microvillar channels. This figure represents a higher magnifica- tion view of the microvillar compart- ment of luteal cells immunostained for SR-BI. A shows this cell compartment after incubation with a preimmune se- rum (1:100), and B shows a similar sec- tion that had been incubated with SR-BI [antiserum against the proposed extracellular domain of mouse SR-BI (1: 100)]: these images were purposely overlabeled to illustrate the close asso- ciation of SR-BI with microvillar chan- nels (defined by double arrows).

Article Snippet: Paraffin-prepared sections were blocked with 5% goat serum and 5% nonfat milk (1 h, 37 C), incubated with primary antibody or preimmune serum (1:1000) overnight at 4 C, and labeled with a standard biotinylated horseradish peroxidase procedure (Vector Laboratories, Burlingame, CA).

Techniques: Incubation